Mouse IgG1 Isotype Control

Images

 
SDS-Page: Mouse IgG1 Native Protein [NBP1-96982] - SDS-PAGE of Mouse IgG1 Lambda Isotype Control. Lane 1: Mouse IgG1 Lambda Isotype Control, Reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG1 Lambda Isotype ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, IP, PAGE
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Mouse IgG1 Isotype Control Summary

Description
A full-length protein corresponding to Mouse IgG1.

Source: Serum

Protein/Peptide Type
Native Protein
Purity
Antigen and protein A Affinity-purified

Applications/Dilutions

Dilutions
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • SDS-Page 1:100 - 1:2000
Application Notes
Mouse igG lambda isotype control is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Antigen and protein A Affinity-purified

Alternate Names for Mouse IgG1 Isotype Control

  • IgG1

Background

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda. IgG1 is the most abundant of the four IgG subclasses. This isotype control possesses lambda light chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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