Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [Allophycocyanin]

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Flow Cytometry: Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [Allophycocyanin] [NBP2-30344APC] - Intracellular flow analysis of KLF4 expressed by mouse splenocytes using 1 ug (red) and 1 ug of isotype control ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Allophycocyanin

Order Details

Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [Allophycocyanin] Summary

Immunogen
Goat Anti-Rabbit IgG APC conjugated secondary antibody (F(ab')2-specific)
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 ul/10^6 cells (0.25ug)
Application Notes
The antibody conjugate has been tested by FACS analysis at 0.5 ul / one million cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.
Publications
Read Publication using
NBP2-30344APC in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24518205)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Antibody in 100 ul PBS containing 15 mg/ml BSA.
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.5 ml deionized water to desired concentration.

Background

Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Minimal cross-reaction to human, mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-30344APC)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow-IC.


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