Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE]


Flow Cytometry: Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE] [NBP2-30343PE] - Ki-67 expression in actively growing Jurkat cells: Cells were stained with 0.2 ug of Ki-67 antibody (red) and isotype control ...read more

Product Details

Reactivity RbSpecies Glossary
Applications Flow
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Goat F(ab')2 anti-Rabbit IgG (H L) Secondary Antibody [PE] Summary

Goat Anti-Rabbit IgG (H+L) PE conjugated secondary antibody
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C in the dark.
PE (A=488, E=575)
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 containing 15 mg BSA/ml
0.05% Sodium Azide
Immunogen affinity purified


  • Flow Cytometry 0.5 ul/10^6 cells
Application Notes
he antibody conjugate has been tested by FACS analysis at 0.5 ul/10^6 cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.
Read Publications using
NBP2-30343PE in the following applications:

  • 3 publications

Reactivity Notes



Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. PE concentration is 0.5 mg/ml, (determined by absorption = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) Minimal cross-reaction to human, rat, horse and mouse.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-30343PE)(4)

Reviews for IgG (H+L) Antibody (NBP2-30343PE) (0)

There are no reviews for IgG (H+L) Antibody (NBP2-30343PE). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IgG (H+L) Antibody (NBP2-30343PE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG (H+L) Products

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Contact Information

Product PDFs

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-30343PE below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought