Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE]

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Flow Cytometry: Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE] [NBP2-30343PE] - Ki-67 expression in actively growing Jurkat cells: Cells were stained with 0.2 ug of Ki-67 antibody (red) and isotype control ...read more

Product Details

Summary
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    • Catalog Number
      NBP2-30343PE
    • Availability
      Product Discontinued

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Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE] Summary

Immunogen
Goat Anti-Rabbit IgG (H+L) PE conjugated secondary antibody
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 ul/10^6 cells
Application Notes
he antibody conjugate has been tested by FACS analysis at 0.5 ul/10^6 cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-30343PE in the following application:

Publications
Read Publications using
NBP2-30343PE in the following applications:

  • 3 publications

Reactivity Notes

Rabbit

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 containing 15 mg BSA/ml
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. PE concentration is 0.5 mg/ml, (determined by absorption = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) Minimal cross-reaction to human, rat, horse and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-30343PE)(4)

Review for IgG (H+L) Antibody (NBP2-30343PE) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-30343PE:
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Flow Cytometry IgG (H+L) NBP2-30343PE
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reviewed by:
Jim Hsiao
Flow 02/27/2017
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Summary

ApplicationFlow Cytometry
Lot4306318

Comments

CommentsCells were isolated and blocked with Fc block for 30 minutes. Cells were then fixed and permeabilized with BD Perm/fix kit for 30 minutes followed by the incubation of biotinylated-conjugated IL1b antibody (1:100 in PERM buffer) for 30 minutes on ice. Cells were washed and stained with Goat F(ab')2 anti-Rabbit IgG (H+L) Secondary Antibody [PE] (1:100 in PERM buffer) for additional 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.

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Recent Reviews

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Jim Hsiao
02/27/2017
Application: Flow
Species: