Donkey anti-Rat IgG (H+L) Secondary Antibody [FITC]

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Flow Cytometry: Donkey anti-Rat IgG (H+L) Secondary Antibody [FITC] [NBP2-30339F] - Analysis of CD25 in ConA stimulated mouse splenocytes using 0.1 ug/10^6 cells of IMG-5924A-0200. Shaded histogram represents cells ...read more

Product Details

Summary
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Order Details


    • Catalog Number
      NBP2-30339F
    • Availability
      Product Discontinued

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Donkey anti-Rat IgG (H+L) Secondary Antibody [FITC] Summary

Immunogen
Donkey anti-Rat IgG (H+L)-FITC Conjugate
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 ul/10^6 cells
Application Notes
The antibody conjugate has been tested by FACS analysis at 0.70 ug/one million cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.
Publications
Read Publication using NBP2-30339F.

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
300 ug in 250 ul PBS containing 15 mg BSA/ml..
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rat IgG and with the light chains common to most rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and sheep serum proteins, but the antibody may cross-react with immunoglobulins from other species. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Flurophore/Protein: 12 ug/mg; 3.1 moles FITC per mole F(ab)2. Minimal cross-reaction to human, bovine, horse and rabbit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rat IgG, H/L Chains Antibody (NBP2-30339F)(1)


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Calculators

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