Flow Cytometry: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [FITC] [NBP2-30335] - Staining of 10^6 PBMC using 1 ug of CD45RA antibody (red, ABX-119A), 0.5 ul of anti mouse IgG FITC secondary (20102) and mouse ...read more
The antibody conjugate has been tested by FACS analysis at 0.70 ug/one million cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.
Read Publications using NBP2-30335 in the following applications:
Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Flurophore/Protein: 12 mg/mg; 3.1 moles FITC per mole F(ab)2. Minimal cross-reaction to human, bovine, horse and rabbit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IgG (H+L) Antibody (NBP2-30335)(2)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 1 application: ICC/IF.