Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [FITC]

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Flow Cytometry: Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [FITC] [NBP2-30335] - Staining of 10^6 PBMC using 1 ug of CD45RA antibody (red, ABX-119A), 0.5 ul of anti mouse IgG FITC secondary (20102) and mouse ...read more

Product Details

Summary
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    • Catalog Number
      NBP2-30335
    • Availability
      Product Discontinued

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Goat F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [FITC] Summary

Immunogen
Goat Anti-Mouse IgG (H+L)-FITC Conjugate
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
The antibody conjugate has been tested by FACS analysis at 0.70 ug/one million cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.
Publications
Read Publications using
NBP2-30335 in the following applications:

Reactivity Notes

Minimal Cross Reactivity: Human, Bovine, Horse, Rabbit, Swine Serum Proteins

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Purity
Immunogen affinity purified

Background

Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Flurophore/Protein: 12 mg/mg; 3.1 moles FITC per mole F(ab)2. Minimal cross-reaction to human, bovine, horse and rabbit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-30335)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Human
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-30335 Applications Species
Acharya S, Dilnawaz F, Sahoo SK. Targeted epidermal growth factor receptor nanoparticle bioconjugates for breast cancer therapy. Biomaterials. 2009 Oct [PMID: 19631377]
Guchhait SK, Sanghai N, Jain V, Preet R. Combretastatin A-4 Inspired Novel 2-Aryl-3-arylamino-imidazo-pyridines/pyrazines as Tubulin Polymerization Inhibitors, Antimitotic and Anticancer Agents. Med. Chem. Commun. 2014 Mar 17 (ICC/IF, Human) ICC/IF Human

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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