Goat F(ab')2 anti-Rat IgG (H+L) Secondary Antibody [Alkaline Phosphatase]


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Product Details

Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

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Goat F(ab')2 anti-Rat IgG (H L) Secondary Antibody [Alkaline Phosphatase] Summary

Purified Rat IgG (H&L)
Based on IEP, this antibody reacts with: heavy chains on rat IgG and light chains on all rat immunoglobulins. This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species: minimal cross-reactivity with human or mouse IgG
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Alkaline Phosphatase
Alkaline Phosphatase
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
0.05% Sodium Azide
Immunogen affinity purified


  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
  • Immunohistochemistry-Paraffin
Application Notes
This conjugate is suitable for all immunoassay applications.

Reactivity Notes

Rat,Based on IEP, no reactivity is observed to non-immunoglobulin mouse serum proteins; IgG from human or mouse.


Purity > 90% based on SDS-PAGE Small amounts of intact IgG may be present.. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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