Donkey anti-Sheep IgG (H+L) Secondary Antibody

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Western Blot: Donkey anti-Sheep IgG (H+L) Secondary Antibody [NBP1-73953] - Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Sheep antibody at 1,000 overnight at 4C. Secondary antibody: ...read more

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-73953
    • Availability
      Product Discontinued

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Donkey anti-Sheep IgG (H+L) Secondary Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Sheep IgG whole molecule
Specificity
Sheep IgG (H&L) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum. This antibody will detect intact and immunoglobulin components from Sheep.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 5.0ml deionized water to a concentration of 10.0mg/ml.

Notes

Restore with 5.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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