Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed)

Images

 
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NBP1-73240] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Format
Pre-adsorbed
Concentration
LYOPH

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:40000 - 1:80000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Frozen 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
This antibody is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP1-73240 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.0 ml of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-73240) (0)

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Review for IgG (H+L) Antibody (NBP1-73240) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-73240:
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Images Ratings Applications Species Date Details
 
reviewed by:
Kerstin Knies
WB Human 10/23/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate of human fibroblasts
SpeciesHuman

Blocking

Blocking Detailsmilk powder /PBS-T

Primary Anitbody

Dilution Ratio1:2000

Secondary Antibody

Secondary Descriptiongoat anti Mouse
Secondary Manufacturer Cat#73240
Secondary Concentration1:10000

Details

Detection NotesECL, 2 minutes

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Array NBP1-73240

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Kerstin Knies
10/23/2014
Application: WB
Species: Human