Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Images

 
Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NBP1-73240] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
LYOPH

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG, whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:40000 - 1:80000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
Suitable for immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP1-73240 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG (H+L) Antibody (NBP1-73240) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-73240:
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reviewed by:
Kerstin Knies
WB Human 10/23/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate of human fibroblasts
SpeciesHuman

Blocking

Blocking Detailsmilk powder /PBS-T

Primary Anitbody

Dilution Ratio1:2000

Secondary Antibody

Secondary Descriptiongoat anti Mouse
Secondary Manufacturer Cat#73240
Secondary Concentration1:10000

Details

Detection NotesECL, 2 minutes

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Kerstin Knies
10/23/2014
Application: WB
Species: Human