Rabbit anti-Equine IgG (H+L) Secondary Antibody [Cy3]

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Product Details

Summary
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    • Catalog Number
      NBP1-73018
    • Availability
      Product Discontinued

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Rabbit anti-Equine IgG (H+L) Secondary Antibody [Cy3] Summary

Immunogen
Equine IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Horse IgG and Horse Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
Application Notes
This product is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Antiserum concentration: 1.0 mg/mL by UV absorbance at 280 nm

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.5 ml deionized water.

Notes

Emission Wavelength: 565, Excitation Wavelength: 552, Label Molecular Weight: 949, Restore with 0.25 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Additional IgG (H+L) Products

IgG (H+L) NBP1-73018

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