Goat anti-Mouse IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Alkaline Phosphatase
Format
Pre-adsorbed
Concentration
1 mg/ml

Goat anti-Mouse IgG (H+L) Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed) Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:4000
  • ELISA 1:20000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Frozen 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
Application Notes
This product is available in a variety of formats. Anti IgG secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other Alkaline Phosphatase antibody based assays.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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