Goat anti-Chicken IgG (H+L) Secondary Antibody [DyLight 680]

Images

 
Western Blot: Goat anti-Chicken IgY, H/L Chains Antibody [DyLight 680] [NBP1-72562] - Lane 1: Chicken IgG. Lane 2: none. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: DyLight (TM) 680 chicken ...read more
Fluorophore-linked immunosorbent assay: Goat anti-Chicken IgY, H/L Chains Antibody [DyLight 680] [NBP1-72562]

Product Details

Summary
Reactivity ChSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 680
Concentration
LYOPH

Order Details

Goat anti-Chicken IgG (H+L) Secondary Antibody [DyLight 680] Summary

Immunogen
Chicken IgY, whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. This antibody will react with heavy chains of Chicken IgG and Chicken IgY and with light chains of most Chicken immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
DyLight Dye Conjugated Chicken Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 715, Excitation Wavelength: 682, Label Molecular Weight: 950, Restore with 1.0 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGHG