Goat anti-Mouse IgG (H L) Secondary Antibody [Cy3] (Pre-absorbed)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Cy3
Format
Pre-absorbed

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Goat anti-Mouse IgG (H+L) Secondary Antibody [Cy3] (Pre-absorbed) Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Publications
Read Publication using NB7607.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Background

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB7607)(1)

We have publications tested in 2 applications: IHC-Fr, IHC-FrFl.


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IHC-Fr
(1)
IHC-FrFl
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG Antibody (NB7607). (Showing 1 - 1 of 1 FAQ).

  1. Could you suggest me which is your best secondary antibody goat anti mouse Cy3-conjugated to be used for IHC or ICC?
    • In regards to your inquiry we have two secondary goat anti mouse IgG antibodies that have been validated for IF. Our IF application applies to ICC. Neither has been validated for IHC, but as they are just secondary if your primary is present they should work just fine. We guarantee both for IF/ICC applications and if you want a specific recommendation I would choose the one that is lower in price as they should both work just as well.

Additional IgG (H+L) Products

IgG (H+L) NB7607

Blogs on IgG (H+L)

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