Goat anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase]

Product Discontinued

Goat anti-Rabbit IgG (H+L) Secondary Antibody [Alkaline Phosphatase] Summary

• Immunogen: Rabbit IgG. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to alkaline phosphatase (alkphos).
• Localization: Secreted
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against nonimmunoglobulin serum proteins. This may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:1000 - 1:10000
• Application Notes: ELISA, immunohisto/cytochemistry, and Western blotting.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB730-AP)(3)

Publications using NB730-AP

• Drobnak I, Braselmann E, Clark PL. Multiple Driving Forces Required for Efficient Secretion of Autotransporter Virulence Proteins J. Biol. Chem. 2015-02-10 [PMID: 25670852] (ELISA)
• Yeung PL, Denissova NG, Nasello C, Hakhverdyan Z, Chen JD, Brenneman MA. Promyelocytic leukemia nuclear bodies support a late step in DNA double-strand break repair by homologous recombination. J Cell Biochem;113(5):1787-99. 2012-05-01 [PMID: 22213200] (ICC/IF, Rabbit)
• Korajoki H, Vornanen M. Expression of calsequestrin in atrial and ventricular muscle of thermally acclimated rainbow trout. J Exp Biol;212(Pt 21):3403-14. 2009-11-01 [PMID: 19837881] (WB, Rabbit)

Review for IgG Antibody (NB730-AP) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 03/02/2009

Summary

• Application: Western Blot
• Sample Tested: recombinant protein, Sample Amount: 0.5 ug
• Species: Other
• Lot: P14