Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP]

Product Discontinued

Rabbit anti-Goat IgG (H+L) Secondary Antibody [HRP] Summary

• Immunogen: Goat IgG.
• Localization: Scereted
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 to 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:30000
• Application Notes: ELISA, Western Blot, Colorimetric
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Reacts with goat. Human reactivity reported in scientific literature (PMID: 25303791)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.1% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB710-H)(6)

Publications using NB710-H

• Nahalkova J, Tomkinson B. TPPII, MYBBP1A and CDK2 form a protein-protein interaction network. Arch. Biochem. Biophys. 2014-10-07 [PMID: 25303791] (WB)
• Long Eric K, Olson Dalay M, Bernlohr David A et al. High-fat diet induces changes in adipose tissue trans-4-oxo-2-nonenal and trans-4-hydroxy-2-nonenal levels in a depot-specific manner. Free Radical Biology & Medicine 2013-01-01 [PMID: 23726997] (WB, WB)
• Makinde TO, Agrawal DK. Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice. Am J Respir Cell Mol Biol. 2010-05-12 [PMID: 20463289] (ELISA)
• Balin Samuel J, Cascalho Marilia, . The rate of mutation of a single gene. Nucleic Acids Res 2010-01-01 [PMID: 20007603] (WB)
• Grimsey N, Han G-S, O'Hara L et al. Temporal Spatial Regulation of the Phosphatidate Phosphatases Lipin 1 2. J Biol Chem;283(43):29166-29174. 2008-01-01 [PMID: 18694939]
• Fawcett KA, Grimsey N, Loos RJF et al. Evaluating the Role of LPIN1 Variation in Insulin Resistance, Body Weight, Human Lipodystrophy in U. K Populations Diabetes;57(9):2527-2533. 2008-01-01 [PMID: 18591397] (WB)

Review for IgG Antibody (NB710-H) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/02/2014

Summary

• Application: Western Blot
• Sample Tested: Jurkat cells
• Species: Human