Goat anti-Mouse IgG (H+L) Secondary Antibody [HiLyte Fluor 488]

Product Discontinued

Goat anti-Mouse IgG (H+L) Secondary Antibody [HiLyte Fluor 488] Summary

• Immunogen: Mouse IgG
• Specificity: Mouse IgG (H+L)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence

Reactivity Notes

Reacts with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.01 mM phosphate, 150 mM NaCl, pH 7.2, with bovine serum albumin.
• Preservative: 2mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

HiLyte Fluor(TM) 488-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. HiLyte Fluor(TM)488 is a green flurophore with brighter fluorescence and better photostability than fluorescein, such as FITC. The spectrum of HiLyte Fluor(TM)488 is similar to FITC, resulting in an optimal match to filters designed for fluorescein. The fluorescence of HiLyte Fluor(TM) 488 can be observed at excitation/emission wavelength of 499 nm/523 nm.The goat anti-mouse IgG is highly adsorbed with minimal cross-reaction to human, bovine, horse, rabbit, and swine serum proteins. This secondary antibody is ideal for multi-fluorescence staining in which several primary antibodies from different species are involved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.