Goat anti-Rabbit IgG (H+L) Secondary Antibody [HiLyte Fluor 488]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB710-94932
    • Availability
      Product Discontinued

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [HiLyte Fluor 488] Summary

Immunogen
Rabbit IgG
Specificity
This IgG is highly adsorbed with minimal cross-reaction to human, mouse, and rat serum protein
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1-10 ug/ml
Publications
Read Publication using NB710-94932.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM phosphate, 150 mM NaCl, pH 7.2, with BSA
Preservative
2mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Background

HiLyte Fluor(TM) 488-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. HiLyte Fluor(TM)488 is a green flurophore with brighter fluorescence and better photostability than fluorescein, such as FITC. The spectrum of HiLyte Fluor(TM)488 is similar to FITC, resulting in an optimal match to filters designed for fluorescein. The fluorescence of HiLyte Fluor(TM) 488 can be observed at excitation/emission wavelength of 499 nm/523 nm.The goat anti-rabbit IgG is highly adsorbed with minimal cross-reaction to human, mouse, and rat serum protein. This secondary antibody is ideal for multi-fluorescence staining in which several primary antibodies from different species are involved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB710-94932)(1)

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ICC/IF Video Protocol

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