Rabbit anti-Human IgG (H+L) Secondary Antibody [Biotin]

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Western Blot: Rabbit anti-Human IgG (H+L) Secondary Antibody [Biotin] [NB120-7159] - Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human IgG Antibody biotin conjugated at 1:1,000 for 60 min at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

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Catalog# & Formulation Size Price

Rabbit anti-Human IgG (H+L) Secondary Antibody [Biotin] Summary

Immunogen
Human IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2-10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free.
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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