Rabbit anti-Human IgG (H+L) Secondary Antibody [Biotin]


Western Blot: Rabbit anti-Human IgG (H+L) Secondary Antibody [Biotin] [NB120-7159] - Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human IgG Antibody biotin conjugated at 1:1,000 for 60 min at ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Rabbit anti-Human IgG (H L) Secondary Antibody [Biotin] Summary

Human IgG whole molecule
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2-10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free.
0.05% Sodium Azide
Immunogen affinity purified


  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
Readout System


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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