Goat anti-Chicken IgG (H+L) Secondary Antibody [Cy2] (Pre-absorbed)

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Western Blot: Goat anti-Chicken IgY Secondary Antibody [Cy2] [NB120-6960] - Western Blot of Anti-Chicken IgG (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins). Lane M: 3 ul Molecular Ladder. Lane ...read more

Product Details

Summary
Reactivity ChSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Cy2
Format
Pre-absorbed

Goat anti-Chicken IgG (H+L) Secondary Antibody [Cy2] (Pre-absorbed) Summary

Immunogen
Full length native Chicken IgY (purified).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500-1:2500
  • Fluorophore-linked immunosorbent assay 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000

Reactivity Notes

Chicken.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/ml Bovine Serum
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGHG