Donkey anti-Goat IgG (H+L) Secondary Antibody [Cy2] (Pre-absorbed)

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Western Blot: Donkey anti-Goat IgG Secondary Antibody [Cy2] [NB120-6948] - Western Blot of Donkey anti-Goat IgG Cy2 Conjugated Antibody. Lane 1: Goat IgG. Lane 2: None. Load: 50 ng.Primary antibody: ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
Cy2
Format
Pre-absorbed

Donkey anti-Goat IgG (H+L) Secondary Antibody [Cy2] (Pre-absorbed) Summary

Immunogen
Full length native Goat IgG (purified).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
Flow Cytometry Use at an assay dependant concentration. ICC: Use at an assay dependant concentration. IF: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IHC-Fr: Use at an assay dependant concentration.

Reactivity Notes

Minimum Cross Reactivity to Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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