Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 680]

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
DyLight 680

Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 680] Summary

Immunogen
This Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody was developed against goat IgG whole molecule.
Specificity
Assay of Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot : 1:2000 - 1:10000
  • ELISA : 1:20000 - 1:100000
  • Immunohistochemistry : 1:1000 - 1:5000
  • Immunohistochemistry-Frozen : 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin : 1:1000 - 1:5000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

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Other Available Formats

Allophycocyanin/Cy7 NB120-6520APCCY7
Biotin NB120-6578
DyLight 405 NB120-6520V
DyLight 488 NB120-6520G
DyLight 550 NB120-6520R
DyLight 594 NB120-6520DL594
DyLight 650 NB120-6520C
DyLight 680 NB120-6520FR
DyLight 755 NB120-6520IR
FITC NB120-6521
HRP NB120-6667
Janelia Fluor 549 NB120-6520JF549
Janelia Fluor 646 NB120-6520JF646
PE/Atto594 NB120-6520PEATT594
PE/Cy5.5 NB120-6520PECY55
PE/Cy7 NB120-6520PECY7
Rhodamine NB120-6522
Texas Red NB120-6523

Additional IgG (H+L) Products

Array NB120-6520FR

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