Canine IgG Fc Isotype Control

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Host
Canine
Conjugate
Unconjugated
Format
Purified
Concentration
1.0 mg/ml

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Canine IgG Fc Isotype Control Summary

Description
Native Canine IgG, Fc.
Specificity
Canine IgG F(c) Fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Canine Serum, anti-Canine IgG and anti-Canine IgG F(c). No reaction was observed against anti-Canine IgG F(ab)2 or anti-Papain.
Isotype
IgG
Host
Canine
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ion exchange chromatography

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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