Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [PE]


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Product Details

Reactivity RtSpecies Glossary
Applications Flow, ICC/IF
0.5 mg/ml

Order Details

Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [PE] Summary

Rat IgG F(c) fragment
ssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PE (A=488, E=575)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA)
0.05% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


Application Notes
Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required - stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 - 1:250 should be suitable for most applications.

Reactivity Notes

Cross-reacts with Rat.Does not cross-react with Cow, Horse or Human.Not yet tested in other species.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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IgG Fc fragment NB120-6259

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