Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [PE] (Pre-absorbed)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
PE
Format
Pre-absorbed
Concentration
0.5 mg/ml

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Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [PE] (Pre-absorbed) Summary

Immunogen
Rat IgG F(c) fragment
Specificity
ssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:250
Application Notes
Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required - stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 - 1:250 should be suitable for most applications.

Reactivity Notes

Cross-reacts with Rat.Does not cross-react with Cow, Horse or Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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