Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [Alkaline Phosphatase] (Pre-absorbed)



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Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [Alkaline Phosphatase] (Pre-absorbed) Summary

Rat IgG F(c) fragment
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F(ab')2 or Bovine, Horse, and Human Serum Proteins.
Immunogen affinity purified


  • Western Blot 1:500 - 1:2500
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
Application Notes
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 - 1:5,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross-reacts with Rat. Does not cross-react with Cow, Horse or Human. Not yet tested in other species.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml BSA (Immunoglobulin and Protease free)
0.05% Sodium Azide
Immunogen affinity purified


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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