Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [Alkaline Phosphatase]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-6258
    • Availability
      Product Discontinued

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Goat F(ab')2 anti-Rat IgG Fc fragment Secondary Antibody [Alkaline Phosphatase] Summary

Immunogen
Rat IgG F(c) fragment
Localization
Secreted
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F(ab')2 or Bovine, Horse, and Human Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2500
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
Application Notes
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 - 1:5,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross-reacts with Rat. Does not cross-react with Cow, Horse or Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml BSA (Immunoglobulin and Protease free)
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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