Goat anti-Human IgG Fc Secondary Antibody [DyLight 649] (Pre-absorbed)



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Goat anti-Human IgG Fc Secondary Antibody [DyLight 649] (Pre-absorbed) Summary

Human IgG-Fc Fragment
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight(R) 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
Immunogen affinity purified


  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500
Application Notes
Useful in Flow cytometry / FACS analysis, Immunocytochemistry and Immunohistochemistry-p.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS containing 0.2% BSA
0.09% Sodium Azide
Immunogen affinity purified


DyLight 649 is excited at 646 (in PBS) and emits at 674 (in PBS).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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