Goat anti-Human IgG F(ab) Secondary Antibody [HRP]


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Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
0.5 mg/ml

Order Details

Goat anti-Human IgG F(ab) Secondary Antibody [HRP] Summary

Human IgG-F(ab')2 Fragment
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Phosphate Buffered Saline (PBS) containing 0.2%BSA
0.1% Pro-Clean 400
0.5 mg/ml
Immunogen affinity purified


Antiserum was solid phase adsorbed to ensure specificity. The antibody to human F(ab')2 was isolatedby affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generatedusing a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species. Suggested starting dilutions are: ELISA Colorimetric: 1:2000 - 1:20000, Chemiluminescent: 1:5000 - 1:25000


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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