Golden Syrian Hamster IgG F(ab)2 Isotype Control

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Product Details

Summary
Reactivity SyHaSpecies Glossary
Applications WB, ELISA
Host
Golden Syrian Hamster
Conjugate
Unconjugated
Format
Purified
Concentration
1.0 mg/ml

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Golden Syrian Hamster IgG F(ab)2 Isotype Control Summary

Description
Native Golden Syrian Hamster IgG, Fab2.

Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Golden Syrian Hamster IgG, anti-Golden Syrian Hamster IgG F(ab)2 and anti-Golden Syrian Hamster Serum. No reaction was observed against anti-Golden Syrian Hamster IgG F(c) or anti-Pepsin.
Isotype
IgG
Host
Golden Syrian Hamster
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ion exchange chromatography

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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