Goat anti-Mouse IgG Secondary Antibody [HRP]

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Western Blot: Goat anti-Mouse IgG Secondary Antibody [HRP] [NBP2-30347H] - Analysis of anti-TRF2 antibody (cat # IMG-124) using Imgenex's anti-mouse HRP (cat # 20101) at 1:5000.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
HRP

Order Details

Goat anti-Mouse IgG Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG + IgM (H + L)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • ELISA 1:1000-1:10000
Application Notes
This product does not contain sodium azide. Sodium azide is an inhibitor of peroxidase.
Readout System
Publications
Read Publications using
NBP2-30347H in the following applications:

  • WB
    3 publications

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
100 ul antibody in PBS with 15 mg/ml BSA
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [HRP]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NBP2-30347H)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (NBP2-30347H) (0)

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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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