Rabbit F(ab')2 anti-Goat IgG Secondary Antibody [Fluorescein]

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Fluorescein
Concentration
LYOPH

Rabbit F(ab')2 anti-Goat IgG Secondary Antibody [Fluorescein] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Goat IgG F(ab')2 fragment
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum. No reaction was observed against Goat IgG F(c).
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000

Reactivity Notes

Reacts with Goat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 2.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit F(ab')2 anti-Goat IgG Secondary Antibody [Fluorescein]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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