This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
Immunogen affinity purified
Western Blot 1:1000 - 1:10000
ELISA 1:10000 - 1:50000
Immunohistochemistry 1:500 - 1:2500
Immunohistochemistry-Paraffin 1:500 - 1:2500
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:2,000 - 1:12,000 is suggested for this product.
FAQs for IgG Antibody (NBP1-73640). (Showing 1 - 1 of 1 FAQ).
I have couple of questions for you about your NBP1-73640 antibody. What exactly is this antibody? Is this antibody a Fab2 fragment only? And the data sheet says that Mouse IgG F(ab')2 fragment was used as an immunogen. When using Mouse IgG F(ab')2 fragment as an immunogen what does the antibody recognize versus when using Mouse IgG F(c) fragment as an immunogen? Are there some differences? I would like to have anti-Mouse IgG antibody that is IgG F(ab')2 fragment conjugated with HRP.
NBP1-73640 is affinity purified whole rabbit IgG which binds to the F(ab)2 part of mouse IgG. IgG normally is composed of two longer heavy chains with two shorter light chains in a Y shape. The arms of the Y are composed of both heavy and light chains and are the F(ab)2 or Fab fragments. The stem of the Y is the F(C) portion formed from the lower part of the heavy chains.
Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains... Read full blog post.
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