Rabbit F(ab')2 anti-Mouse IgG Secondary Antibody [HRP]

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Western Blot: Rabbit F(ab')2 anti-Mouse IgG Secondary Antibody [HRP] [NBP1-73640] - WB shows detection of mouse anti-beta Actin monoclonal antibody (Catalog# NBP1-47423) using a HRP conjugated rabbit anti-mouse IgG, ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP
Concentration
LYOPH

Rabbit F(ab')2 anti-Mouse IgG Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG F(ab')2 fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:2,000 - 1:12,000 is suggested for this product.
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Reviewed Applications
Read 1 Review rated 5
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NBP1-73640 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit F(ab')2 anti-Mouse IgG Secondary Antibody [HRP]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG Antibody (NBP1-73640) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-73640:
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Images Ratings Applications Species Date Details
Western Blot IgG NBP1-73640
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reviewed by:
Anonymous
WB Human 10/08/2015
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Summary

ApplicationWestern Blot
Sample Testedhuman cancer cell lysate
SpeciesHuman
Lot12112

Blocking

Blocking Details5% non-fat milk in TBS buffer, room temperature for 1 hour

Primary Anitbody

Dilution Ratio1:3000 in 5% non-fat milk in TBS buffer overnight at 4C

Secondary Antibody

Secondary Descriptionanti-Mouse IgG, Fab2 Antibody
Secondary Manufacturer Cat#NBP1-73640
Secondary Concentration1:5000

Details

Detection Notes1 min exposure show strong band.

Comments

CommentsThis anti-mouse IgG Fab antibody is very specific to Fab2. In my test, I use Fc-fusion protein and this antibody does not react to Fc.

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FAQs for IgG Antibody (NBP1-73640). (Showing 1 - 1 of 1 FAQ).

  1. I have couple of questions for you about your NBP1-73640 antibody. What exactly is this antibody? Is this antibody a Fab2 fragment only? And the data sheet says that Mouse IgG F(ab')2 fragment was used as an immunogen. When using Mouse IgG F(ab')2 fragment as an immunogen what does the antibody recognize versus when using Mouse IgG F(c) fragment as an immunogen? Are there some differences? I would like to have anti-Mouse IgG antibody that is IgG F(ab')2 fragment conjugated with HRP.
    • NBP1-73640 is affinity purified whole rabbit IgG which binds to the F(ab)2 part of mouse IgG. IgG normally is composed of two longer heavy chains with two shorter light chains in a Y shape. The arms of the Y are composed of both heavy and light chains and are the F(ab)2 or Fab fragments. The stem of the Y is the F(C) portion formed from the lower part of the heavy chains.

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Anonymous
10/08/2015
Application: WB
Species: Human