Rabbit F(ab')2 anti-Human IgG Secondary Antibody [Alkaline Phosphatase]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alkaline Phosphatase
Concentration
1.4 mg/ml

Rabbit F(ab')2 anti-Human IgG Secondary Antibody [Alkaline Phosphatase] Summary

Immunogen
Human IgG F(ab')2 fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2500
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
Application Notes
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,500 - 1:11,000 of the stated concentration is suggested for this product.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA- Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
1.4 mg/ml
Purity
Immunogen affinity purified

Notes

Label Molecular Weight: 140,000

Alternate Names for Rabbit F(ab')2 anti-Human IgG Secondary Antibody [Alkaline Phosphatase]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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