Goat anti-Human IgG Secondary Antibody [HRP]



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Product Discontinued
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Goat anti-Human IgG Secondary Antibody [HRP] Summary

The antibody reacts with the Human IgG antigen.
Antibodies were raised in goat to human IgG. From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepha­rose-bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound. The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtation and ion-exchange chromatography to clear unbound conjuga­te.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No Preservative
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
Recommended dilution as secondary antibody in ELISA, 1:1000. Recommended dilution as secondary antibody in dot-blotting, 1:100.

Reactivity Notes



This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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