Rabbit anti-Human IgG Secondary Antibody [FITC]

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Product Details

Summary
Product Discontinued
View other related IgG Secondary Antibodies

Order Details


    • Catalog Number
      NBP1-42097
    • Availability
      Product Discontinued

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Rabbit anti-Human IgG Secondary Antibody [FITC] Summary

Immunogen
The antibody reacts with the Human IgG antigen.
Specificity
Antibodies were raised in rabbit to human IgG. From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound. The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate. F/P ratio: 4-6 mol Fluorescein per 1 mol of rabbit IgG. Low background fluorescence of normal tissue in contrast to cases of autoimmune desease, depostited immunoglobulin IgG. No cross reactivity with albumine fractions of human serum. No cross reactivity withalbumine fractions of animal species.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
For immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Rabbit anti-Human IgG Secondary Antibody [FITC]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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