Goat anti-Mouse IgG Secondary Antibody [Cy3]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Cy3

Order Details

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Goat anti-Mouse IgG Secondary Antibody [Cy3] Summary

Immunogen
Full length native Mouse IgG (purified).
Specificity
Mouse IgG Heavy and Light
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:500-1:2500
  • Fluorophore-linked immunosorbent assay 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
Publications
Read Publication using NB7607.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [Cy3]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB7607)(1)

We have publications tested in 2 applications: IHC-Fr, IHC-FrFl.


Filter By Application
IHC-Fr
(1)
IHC-FrFl
(1)
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Filter By Species
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Antibody (NB7607). (Showing 1 - 1 of 1 FAQ).

  1. Could you suggest me which is your best secondary antibody goat anti mouse Cy3-conjugated to be used for IHC or ICC?
    • In regards to your inquiry we have two secondary goat anti mouse IgG antibodies that have been validated for IF. Our IF application applies to ICC. Neither has been validated for IHC, but as they are just secondary if your primary is present they should work just fine. We guarantee both for IF/ICC applications and if you want a specific recommendation I would choose the one that is lower in price as they should both work just as well.

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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