Goat anti-Mouse IgG Secondary Antibody [Cy5]

Images

 
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG Antibody [Cy5] [NB7602]
Immunohistochemistry-Frozen: Goat anti-Mouse IgG Antibody [Cy5] [NB7602] - analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.
Flow Cytometry: Goat anti-Mouse IgG Antibody [Cy5] [NB7602]
Immunohistochemistry-Paraffin: Goat anti-Mouse IgG Antibody [Cy5] [NB7602] - analysis of paraffin embedded human colonic tissue using Goat anti-Mouse IgG [Cy5] secondary antibody. Image from verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications FLISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Cy5
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

Goat anti-Mouse IgG Secondary Antibody [Cy5] Summary

Immunogen
Mouse IgG, whole molecule (H&L)
Specificity
Mouse IgG (H and L)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500-1:2500
  • Fluorophore-linked immunosorbent assay 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
Application Notes
Suitable for immunomicroscopy and flow cytometry or analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB7602 in the following applications:

Reactivity Notes

No reaction is observed against Bovine, Chicken, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent) to desired concetration.

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [Cy5]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species (human labelled with the fluorophore Cy5 here) in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB7602) (0)

There are no publications for IgG Antibody (NB7602).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IgG Antibody (NB7602) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 3 application: IHC-P, Flow, ICC.

Reviews using NB7602:
Filter by Applications
IHC-P
(1)
Flow
(1)
ICC
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin IgG NB7602
Enlarge
reviewed by:
Arun Satelli
IHC-P Human 11/04/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman colonic tissue
SpeciesHuman
CommentsVery good secondary to detect in IHC paraffin section.

Blocking

Blocking DetailsFish Gelatin

Details

Detection NotesDetection using confocal microscope LSM510

Comments

CommentsVery good secondary to detect in IHC paraffin section.
Flow Cytometry IgG NB7602
Enlarge
reviewed by:
Arun Satelli
Flow Human 10/16/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman colon cancer cell line HT29
SpeciesHuman

Blocking

Blocking Details0.25% FBS in PBS

Details

Detection NotesDetection using flow cytometer
Fixation DetailsNo fixation, live cells stained with e-Cad first then secondary cy5
Wash Description5 min each 3 times in PBS
Immunocytochemistry IgG NB7602
Enlarge
reviewed by:
Arun Satelli
ICC Human 10/16/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman colon cancer cell line HT29
SpeciesHuman
CommentsAt 1:500, there is sustained view of E-Cadherin in the cells, however, if used at lower concentrations there is non-specific interactions that show up in all cells.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:500

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsCells were fixed in 4% PFA, PBS, Overnight 4 deg C
Wash Description5 min each 3 times in PBS

Comments

CommentsAt 1:500, there is sustained view of E-Cadherin in the cells, however, if used at lower concentrations there is non-specific interactions that show up in all cells.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG Antibody (NB7602) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.3
5
2
4
0
3
1
2
0
1
0

Arun Satelli
11/04/2014
Application: IHC-P
Species: Human

Arun Satelli
10/16/2014
Application: Flow
Species: Human

Arun Satelli
10/16/2014
Application: ICC
Species: Human