Chicken anti-Rabbit IgG Secondary Antibody [HRP]

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Chicken
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

Chicken anti-Rabbit IgG Secondary Antibody [HRP] Summary

Immunogen
Chickens were immunized with purified, non-denatured rabbit IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-rabbit IgG's were affinity-purified over a column conjugated with purified rabbit IgG (containing both heavy and light chains) and then conjugated with horseradish peroxidase.
Specificity
Antibodies were tested for specificity using double-immunodiffusion in agarose gels and ELISA analysis. In the latter, a 1:20000 dilution gave an O.D. of 1.0 in a 15 minute reaction using tetramethylbenzidine as substrate.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • ELISA 1:20000
Application Notes
ELISA and Western Blot
Readout System
Publications
Read Publications using
NB710-57836 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

There is no detectable cross-reactivity with goat, human or mouse IgG.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin
Preservative
Thimerosal
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Chicken anti-Rabbit IgG Secondary Antibody [HRP]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB710-57836)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA, WB.


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ELISA
(1)
WB
(1)
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Filter By Species
Human
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Showing Publications 1 - 2 of 2.
Publications using NB710-57836 Applications Species
Florea F, Bernards C, Caproni M et al. Ex Vivo Pathogenicity of Anti-Laminin gamma1 Autoantibodies. Am J Pathol 2014 Feb [PMID: 24300951] (ELISA) ELISA
Holderied A. Einfluss der parietalen Epithelzellen auf die Bowman'sche Kapsel bei diabetischer Nephropathie Thesis. 2014 (WB, Human) WB Human

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Video Protocols

WB Video Protocol

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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