Goat anti-Rat IgG Secondary Antibody [Cy3.5]

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Western Blot: Goat anti-Rat IgG Secondary Antibody [Cy3.5] [NB120-6954] - Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins). Lane M: 3 ul Molecular Ladder. Lane ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Cy3.5

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Goat anti-Rat IgG Secondary Antibody [Cy3.5] Summary

Immunogen
Full length native Rat IgG (purified).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
Application Notes
Suitable for immunomicroscopy and flow cytometry or analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
Publications
Read Publication using
NB120-6954 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free.
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Rat IgG Secondary Antibody [Cy3.5]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB120-6954)(1)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 1 application: Flow.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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