Goat anti-Mouse IgG Secondary Antibody [Cy2]

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Western Blot: Goat anti-Mouse IgG Secondary Antibody [Cy2] [NB120-6944] - Western Blot of Cy2 conjugated Goat anti-Mouse IgG Pre-adsorbed secondary antibody.Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Cy2

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Goat anti-Mouse IgG Secondary Antibody [Cy2] Summary

Immunogen
Full length native Mouse IgG (purified).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
Flow Cytometry Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IHC-Fr: Use at an assay dependant concentration. IM: Use at an assay dependant concentration. May be useful in WB and FLISA. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [Cy2]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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