Sheep anti-Mouse IgG Secondary Antibody [HRP]

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Western Blot: Sheep anti-Mouse IgG Secondary Antibody [HRP] [NB120-6808] - Lane 1: Mouse IgG. Lane 2: None. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Sheep
Conjugate
HRP
Concentration
LYOPH

Order Details

Sheep anti-Mouse IgG Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:10000 - 1:300000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Application Notes
Suitable for immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Readout System
Publications
Read Publications using
NB120-6808 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/ml BSA Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for Sheep anti-Mouse IgG Secondary Antibody [HRP]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB120-6808)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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