Goat F(ab')2 anti-Mouse IgG Secondary Antibody [PE]


Western Blot: Goat F(ab')2 anti-Mouse IgG Secondary Antibody [PE] [NB120-5889] - Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, ICC/IF
0.5 mg/ml

Order Details

Goat F(ab')2 anti-Mouse IgG Secondary Antibody [PE] Summary

Mouse IgG F(ab)2 fragment
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse or Human Serum Proteins.
Immunogen affinity purified


  • Western Blot 1:1000 - 1:10000
  • Flow Cytometry 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:250
Application Notes
Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required - stain 1 x 10E6 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 - 1:250 should be suitable for most applications.

Reactivity Notes

Cross-reacts with Mouse.Does not cross-react with Cow, Horse, Human, Rabbit, Rat or Sheep.Not yet tested in other species.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.05% Sodium Azide
0.5 mg/ml
Immunogen affinity purified

Alternate Names for Goat F(ab')2 anti-Mouse IgG Secondary Antibody [PE]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB120-5889) (0)

There are no publications for IgG Antibody (NB120-5889).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IgG Antibody (NB120-5889) (0)

There are no reviews for IgG Antibody (NB120-5889). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Antibody (NB120-5889) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Products

Blogs on IgG.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Goat F(ab')2 anti-Mouse IgG Secondary Antibody [PE] and receive a gift card or discount.