Mouse F(ab) anti-Human IgG Secondary Antibody (4A11)

Images

 
Western Blot: Mouse anti-Human IgG Secondary Antibody (4A11) [NB500-467] - Fig. 1. Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment (4A11). Lane 1: Normal ...read more
Flow Cytometry: Mouse anti-Human IgG Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE-CyTM7.
Flow Cytometry: Mouse anti-Human IgG Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE.
Flow Cytometry: Mouse anti-Human IgG Secondary Antibody (4A11) [NB500-467] - Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) purified / GAM-APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Clone
4A11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Mouse F(ab) anti-Human IgG Secondary Antibody (4A11) Summary

Immunogen
Purified human IgG.
Specificity
The antibody 4A11 reacts with Fab fragment of human IgG.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Flow Cytometry 1:10-1:1000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Mouse F(ab) anti-Human IgG Secondary Antibody (4A11)

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (NB500-467) (0)

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Other Available Formats

PE Labeled NBP1-44932

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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