IGFBP-5 Antibody

Images

 
Western Blot: IGFBP-5 Antibody [NBP1-50718] - Recombinant human IGFBP-5. Lane 1: 10 ng, Lane 2: 50 ng.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IGFBP-5 Antibody Summary

Immunogen
E. coli expressed human IGF-BP5
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGFBP5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant human IGFBP-5 can be used as a positive control. Other applications have not been determined.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IGFBP-5 Antibody

  • IBP5IBP-5
  • IGF-binding protein 5
  • IGFBP5
  • IGFBP-5
  • insulin-like growth factor binding protein 5
  • insulin-like growth factor-binding protein 5

Background

IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 over expressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IGFBP5
Entrez
Uniprot