Recombinant Human IGFBP-1 Protein

Product Discontinued

Recombinant Human IGFBP-1 Protein Summary

• Description: A recombinant protein corresponding to 218 amino acids of Human IGFBP-1
• Preparation Method: >98% SDS-PAGE
• Details of Functionality: Biological Activity: The ED50 as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100 ng/ml.
  Results: ~100 ng/ml
• Protein/Peptide Type: Recombinant Protein
• Gene: IGFBP1
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Theoretical MW: 28.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and10 mM HCl.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with water to a final concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4C for 1 week or -20C for future use.

Notes

The purity of this protein is >95% pure by SDS-PAGE

Alternate Names for Recombinant Human IGFBP-1 Protein

• alpha-pregnancy-associated endometrial globulin
• amniotic fluid binding protein
• binding protein-25
• binding protein-26
• binding protein-28
• growth hormone independent-binding protein
• hIGFBP-1
• IBP1
• IBP-1
• IGF-binding protein 1
• IGFBP1
• IGFBP-1
• IGF-BP25
• insulin-like growth factor binding protein 1
• insulin-like growth factor-binding protein 1
• Placental protein 12
• PP12AFBP

Background

Human IGF-BP1 is a 25.4 kDa cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid. Levels of IGF-BP1 in serum are lowest after food. IGF-BP1 binds both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a single, glycosylated protein consisting of 218 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGFBP1