IGF-I R Antibody

Images

 
Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.
Immunocytochemistry/ Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Eq, Pm, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.09 mg/ml

IGF-I R Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human IGF1 Receptor protein (between residues 700-800) [UniProt P08069]
Localization
Membrane.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGF1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
Application Notes
This IGFR1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~100 kDa.
Control Peptide
IGF-I R Peptide (NBP1-77680PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77680 in the following applications:

Publications
Read Publication using
NBP1-77680 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, rat, mouse and primate. Reactivity with Equine reported via customer feedback. Predicted to react with Bovine (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IGF-I R Antibody

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC 2.7.10.1
  • IGF1R
  • IGF-I R
  • IGF-I receptor
  • IGFIR
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2

Background

Insulin-like growth factor receptor 1 (IGFR1) is a tyrosine kinase receptor that is important in embryonic development. IGFR1 has effects in breast, prostate, gastric and lung cancers, as it can be a player in drug resistance. It also affects the flow of nutrients from mother to placenta to fetus during development. IGFR1 seems to greatly influence the rate of growth of fetuses and newborn babies; when it is not appropriately expressed or regulated, fetal growth restriction occurs. The major activity of IGFR1 is to mediate the signaling of growth signals to effect downstream growth patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-I R Antibody (NBP1-77680)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Review for IGF-I R Antibody (NBP1-77680) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77680:
Filter by Applications
WB
(1)
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Filter by Species
Other
(1)
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Images Ratings Applications Species Date Details
Western Blot IGF-I R NBP1-77680
Enlarge
reviewed by:
Christopher England
WB Other 02/04/2016
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Summary

ApplicationWestern Blot
Sample TestedHuman pancreatic cancer cell lines MiaPaCa-2, AsPC-1, and BxPC-3
SpeciesOther
LotA-1

Blocking

Blocking DetailsLiCor Odyssey Blocking Buffer (PBS), 4 hour, room temperature

Primary Anitbody

Dilution RatioDilution 1:1000, 8 hour, room temperature, LiCor Odyssey Blocking Buffer (PBS) w/0.2% Tween 20

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit 800 CW
Secondary Manufacturer Cat#925-32213
Secondary Concentration1 mg/ mL, Dilution 1:15,000

Details

Detection NotesFluorescence detection, LiCor Odyssey, Scan time 4-6 min, Positive MiaPaCa-2 and Negative BxPC-3

Comments

CommentsWe found this antibody to work well for this particular experiment. In our lab, we only want to make sure that our novel imaging agents are targeted to tumors that express the receptor of interest. The delivery time was fast and the company was very nice. Yes, there are some non-specific bands in the blot, yet this is a common occurrence with polyclonal antibodies.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IGF-I R Antibody (NBP1-77680). (Showing 1 - 1 of 1 FAQ).

  1. Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
    • To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77680AF405
Alexa Fluor 488 Labeled NBP1-77680AF488
Alexa Fluor 647 Labeled NBP1-77680AF647
Alexa Fluor 700 Labeled NBP1-77680AF700
Biotin Labeled NBP1-77680B
DyLight 405 Labeled NBP1-77680V
DyLight 488 Labeled NBP1-77680G
DyLight 550 Labeled NBP1-77680R
DyLight 650 Labeled NBP1-77680C
FITC Labeled NBP1-77680F
HRP Labeled NBP1-77680H

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Bioinformatics Tool for IGF-I R Antibody (NBP1-77680)

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Recent Reviews

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Christopher England
02/04/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol IGF1R
Entrez
Uniprot