Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.
Immunocytochemistry/ Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.
Human, rat, mouse and primate. Reactivity with Equine reported via customer feedback. Predicted to react with Bovine (100%).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for IGF-I R Antibody
insulin-like growth factor 1 receptor
Insulin-like growth factor I receptor
soluble IGF1R variant 1
soluble IGF1R variant 2
Insulin-like growth factor receptor 1 (IGFR1) is a tyrosine kinase receptor that is important in embryonic development. IGFR1 has effects in breast, prostate, gastric and lung cancers, as it can be a player in drug resistance. It also affects the flow of nutrients from mother to placenta to fetus during development. IGFR1 seems to greatly influence the rate of growth of fetuses and newborn babies; when it is not appropriately expressed or regulated, fetal growth restriction occurs. The major activity of IGFR1 is to mediate the signaling of growth signals to effect downstream growth patterns.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Fluorescence detection, LiCor Odyssey, Scan time 4-6 min, Positive MiaPaCa-2 and Negative BxPC-3
We found this antibody to work well for this particular experiment. In our lab, we only want to make sure that our novel imaging agents are targeted to tumors that express the receptor of interest. The delivery time was fast and the company was very nice. Yes, there are some non-specific bands in the blot, yet this is a common occurrence with polyclonal antibodies.
Product General Protocols
View specific protocols for IGF-I R Antibody (NBP1-77680):
FAQs for IGF-I R Antibody (NBP1-77680). (Showing 1 - 1 of 1 FAQ).
Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.
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