IGF-I R Antibody

Images

 
Western Blot: IGF-I R Antibody [NBP1-77679] - Analysis of IGF-1R in 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. SK-BR-3 and 8. COS7
Immunocytochemistry/ Immunofluorescence: IGF-I R Antibody [NBP1-77679] - IGF-1 R antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Simple Western: IGF-I R Antibody [NBP1-77679] - Simple Western lane view shows a specific band for IGF-1 R in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.05 mg/ml

IGF-I R Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human IGF1 Receptor protein (between residues 1300-1367) [UniProt P08069].
Localization
Membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGF1R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
Application Notes
This IGFR1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot where a band is seen ~100 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
HeLa Lysate (NB800-PC1)

Reactivity Notes

Human, mouse, monkey and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.05 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IGF-I R Antibody

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC 2.7.10.1
  • IGF1R
  • IGF-I R
  • IGF-I receptor
  • IGFIR
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2

Background

Insulin-like growth factor receptor 1 (IGFR1) is a tyrosine kinase receptor that is important in embryonic development. IGFR1 has effects in breast, prostate, gastric and lung cancers, as it can be a player in drug resistance. It also affects the flow of nutrients from mother to placenta to fetus during development. IGFR1 seems to greatly influence the rate of growth of fetuses and newborn babies; when it is not appropriately expressed or regulated, fetal growth restriction occurs. The major activity of IGFR1 is to mediate the signaling of growth signals to effect downstream growth patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IGF-I R Antibody (NBP1-77679). (Showing 1 - 1 of 1 FAQ).

  1. Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?
    • To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77679AF405
Alexa Fluor 488 Labeled NBP1-77679AF488
Alexa Fluor 647 Labeled NBP1-77679AF647
Alexa Fluor 700 Labeled NBP1-77679AF700
Biotin Labeled NBP1-77679B
DyLight 405 Labeled NBP1-77679V
DyLight 488 Labeled NBP1-77679G
DyLight 550 Labeled NBP1-77679R
DyLight 650 Labeled NBP1-77679C
FITC Labeled NBP1-77679F
HRP Labeled NBP1-77679H

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Bioinformatics

Gene Symbol IGF1R
Entrez
Uniprot