IGF-I R Antibody (33255) [Unconjugated]

Images

 
Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, SK‑Mel‑28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of ...read more
IGF‑I R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF‑I R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature ...read more
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human IGF‑I R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by ...read more
IGF‑I R was detected in immersion fixed MCF‑7 human breast cancer cell line (positive staining; left panel) and HDLM human Hodgkin's lymphoma cell line (negative staining; right panel) using Mouse ...read more
Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, B/N, ELISA(Cap), ICC/IF
Clone
33255
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

IGF-I R Antibody (33255) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
Glu31-Asn932
Accession # P08069
Specificity
Detects human IGF-I R in sandwich ELISAs and Western blots. Detects mouse IGF-I R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL‑9 R, and rhTGF-beta  RII.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IGF1R
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 3-25 ug/mL
  • Neutralization
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human IGF-I R Biotinylated Antibody (Catalog number BAF391)
Standard: Recombinant Human IGF-I R (Catalog number 391-GR)
Publications
Read Publications using
MAB391 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I R Antibody (33255) [Unconjugated]

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC 2.7.10.1
  • IGF1R
  • IGF-I R
  • IGF-I receptor
  • IGFIR
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2

Background

Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-I R Antibody (MAB391)(15)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 6 applications: ELISA Development, Flow, ICC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
ELISA Development
(2)
Flow
(4)
ICC Frozen
(1)
IHC Paraffin-embedded
(1)
Neut
(4)
WB
(3)
All Applications
Filter By Species
Human
(13)
Bovine
(2)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB391 Applications Species
Y Neuzillet, E Chapeaubla, C Krucker, L De Koning, T Lebret, F Radvanyi, I Bernard-Pi IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer BMC Cancer, 2017;17(1):636. 2017 [PMID: 28882129] (Neut,, Human) Neut Human
Helmout Modjtahedi Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs Int. J. Oncol., 2016;49(5):1825-1838. 2016 [PMID: 27599579] (WB,, Human) WB Human
M Gschweitl, A Ulbricht, CA Barnes, RI Enchev, I Stoffel-St, N Meyer-Scha, J Huotari, Y Yamauchi, UF Greber, A Helenius, M Peter A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes Elife, 2016;5(0):. 2016 [PMID: 27008177] (WB,, Human) WB Human
Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez-Villasenor I, Heath P, Romero I, Ince P, Wharton S Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors. Mol Brain, 2015;8(0):51. 2015 [PMID: 26297026] (Neut,, Human) Neut Human
Cardillo T, Trisal P, Arrojo R, Goldenberg D, Chang C Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro. BMC Cancer, 2013;13(0):170. 2013 [PMID: 23548153] (Flow,, Human) Flow Human
Suh H, Zhao M, Derico L, Choi N, Lee S Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators. J Neuroinflammation, 2013;10(0):37. 2013 [PMID: 23497056] (ICC Frozen,, Human) ICC Frozen Human
Jacques C, Holzenberger M, Mladenovic Z Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity. J. Biol. Chem., 2012;287(18):15100-8. 2012 [PMID: 22399297] (Neut,, Human) Neut Human
Massoner P, Ladurner Rennau M, Heidegger I, Kloss-Brandstatter A, Summerer M, Reichhart E, Schafer G, Klocker H Expression of the IGF axis is decreased in local prostate cancer but enhanced after benign prostate epithelial differentiation and TGF-beta treatment. Am. J. Pathol., 2011;179(6):2905-19. 2011 [PMID: 21983635] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Wang N, Fallavollita L, Nguyen L, Burnier J, Rafei M, Galipeau J, Yakar S, Brodt P Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases. Mol. Ther., 2009;17(7):1241-9. 2009 [PMID: 19367255] (ELISA Development,, Human) ELISA Development Human
Back K, Arnqvist HJ Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes. Growth Horm. IGF Res., 2009;19(2):101-11. 2009 [PMID: 18693051] (ELISA Development,, Human) ELISA Development Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol IGF1R
Uniprot