Recombinant Mouse IGF-I Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43173
    • Availability
      Product Discontinued

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Recombinant Mouse IGF-I Protein Summary

Description
A partial protein corresponding to IGF1.
Preparation
Method
E. coli-expressed amino acids Gly33 - Ala102 (Accession# NP001104746).
Details of Functionality
This reagent has been tested in bioassays using the FDC-P1 cell line. The ED50, measured by the dose-dependent induction of FDC-P1 cell proliferation is typically 2 ng/ml, corresponding to a specific activity of approximately 5 x10^5 Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
IGF1
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Reactivity Notes

This protein was made to the Mouse sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sterile liquid, phosphate buffered saline, pH 7.2, with 1.0% BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for Recombinant Mouse IGF-I Protein

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

Insulin-like growth factor I (IGF-1), also known as somatomedin C, is a member of the insulin-like growth factor family. This protein is expressed in many cell types and is secreted into the blood. Metabolism of glucose, fatty acids, cartilage and bone, as well as growth hormone activity, are all regulated by IGF-1. This protein also plays important roles in Alzheimers disease and tumor pathogenesis. IGF-1 and IGF-2 share 70% sequence identity. Furthermore, mature mouse IGF1 shares 94% and 99% amino acid sequence identity with human and rat IGF1, respectively, and exhibits cross-species activity. Recombinant mouse IGF-1 produced in E.coli is a single, non-glycosylated, polypeptide containing 70 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IGF1
Entrez