Recombinant Human IGF-I/IGF-1 Protein

Product Discontinued

Recombinant Human IGF-I/IGF-1 Protein Summary

• Description: A recombinant protein corresponding to IGF1.
• Preparation Method: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences(IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard.
• Details of Functionality: IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by H-thymidine uptake) is less then 1.0 ng/ml, corresponding to a specific activity of 1x10 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.
• Protein/Peptide Type: Recombinant Protein
• Gene: IGF1
• Purity: SDS-PAGE
• Endotoxin Note: Less than 0.1 ng/ug (IEU/ug) of IGF-I.

Applications/Dilutions

Publications

Read Publication using NBP2-26545 in the following applications: Func 1 publication

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Reconstitute with sterilized 18 M omega-cm water to a final concentration of at least 0.1 mg/ml.

Notes

After reconstituted, dilute further with other aqueous solutions.

Alternate Names for Recombinant Human IGF-I/IGF-1 Protein

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

Belongs to the insulin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IGF-I/IGF-1 Recombinant Protein (NBP2-26545)(1)

Publication using NBP2-26545

• Loddo M, Andryszkiewicz J, Rodriguez-Acebes S et al. Pregnancy-associated plasma protein A regulates mitosis and is epigenetically silenced in breast cancer. J. Pathol. 2014-06-13 [PMID: 24931331] (Func, Human)
  
  Details:
  Figs 2I, 2J, 4C: BT549 mammary gland tumor cells and HMEpC normal mammary cells treated with 100 uM recombinant IGF1.

Bioinformatics

• Gene Symbol: IGF1