IGF-I Antibody

Images

 
Western Blot: IGF-I Antibody [NBP2-16929] - Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 15% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: IGF-I Antibody [NBP2-16929] - SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: IGF-I Antibody [NBP2-16929] - Paraffin-embedded human breast carcinoma. IGF1 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, GP, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IGF-I Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Mouse (93%), Rat (94%), Porcine (96%), Rhesus Macaque (100%), Sheep (96%), Bovine (97%), Guinea Pig (100%), Canine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-16929 in the following applications:

Publications
Read Publication using NBP2-16929.

Reactivity Notes

Xenopus laevis (80%), Rabbit (82%), Chicken (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IGF-I Antibody

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-I Antibody (NBP2-16929)(1)

Review for IGF-I Antibody (NBP2-16929) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-16929:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot IGF-I NBP2-16929
Enlarge
reviewed by:
Andrew Cosgrove
WB Mouse 12/16/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
Lot40807

Blocking

Blocking Details5% BSA

Primary Anitbody

Dilution Ratio1:3000 in TBS-T overnight at 4℃

Secondary Antibody

Secondary DescriptionGoat anti Rabbit IgG( H+L) HRP
Secondary Manufacturer Cat#Jackson IR #111-035-003
Secondary Concentration1:5000

Details

Detection NotesMillipore ECL LS4000 for 60secs

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IGF-I Antibody (NBP2-16929) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IGF-I Products

Bioinformatics Tool for IGF-I Antibody (NBP2-16929)

Discover related pathways, diseases and genes to IGF-I Antibody (NBP2-16929). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IGF-I Antibody (NBP2-16929)

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PTMs for IGF-I Antibody (NBP2-16929)

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Research Areas for IGF-I Antibody (NBP2-16929)

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Blogs on IGF-I

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Contact Information

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Calculators

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Recent Reviews

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1
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Andrew Cosgrove
12/16/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol IGF1
Entrez