IGF-I Antibody

Images

 
Western Blot: IGF-I Antibody [NBP1-52088] - IGF1 Antibody [NBP1-52088] (0.5ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: IGF-I Antibody [NBP1-52088] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IGF-I Antibody Summary

Immunogen
Peptide with sequence C-RSVRAQRHTD corresponding to internal region according to NP_001104754.1, NP_001104755.1, NP_000609.1.
Epitope
C-RSVRAQRHTD
Specificity
This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 15 kDa band observed in human uterus lysates (calculated MW of 15.2 kDa band according to NP_001104754.1).
Publications
Read Publication using NBP1-52088.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine, Porcine, Rabbit.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IGF-I Antibody

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972 [PubMed 4550398]). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986 [PubMed 3455760]; Rosenfeld, 2003 [PubMed 14657423]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-I Antibody (NBP1-52088)(1)

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IGF1
Entrez
Uniprot