Rabbit anti-Rat IgA/IgG/IgM Secondary Antibody [HRP]



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Rabbit anti-Rat IgA/IgG/IgM Secondary Antibody [HRP] Summary

The antibody reacts with the Rat IgG antigen.
Antibodies were raised in rabbit to rat IgG, IgM and IgA. Specific antibodies were absorpted by incubation with Sepharose-bound rat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized rat immunoglobulines a minimum of 65% protein bound. The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate. No cross reaction with albumin fraction of rat serum. No cross reaction with albumin fraction of other animal species. No background was seen on human tissue sections.
Immunogen affinity purified


Application Notes
Recommended dilution as secondary antibody in ELISA, 1:1000. Recommended dilution as secondary antibody in dot-blotting, 1:100.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No Preservative
Immunogen affinity purified


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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